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  • Sperm Analysis Equipment Factory, Sperm Analysis

    Hc-B028 High Quality Laboratory Trolley Type Sperm Analyzer Semen Computer Assisted Sperm Analysis System FOB Price: US 1000-2100 Piece Min. Order: 1 Piece

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    Product Name: Disposable Sperm Counting Chamber/Plate; Properties: Veterinary Medical Materials & Accessories; Analyzing Area: 4, 8 Seperate Testing Chambers; Model Number: 10um, 20um; Function: Sperm Analysis; Material: Glass

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    Product Name: Animal Sperm Quality Analyzer; Applicable Animal: Pig, Sheep, Sheep, Dog, etc. Function: Sperm Quality Analyzer; Screen: Full Touch Screen; USB Interface: Multiple USB Interfaces; Detection Time: 30 Seconds

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    Name: Sperm Counting Chamber/Sperm Count Capsule; Dimensions: 74 * 36 *7mm (Plate with The Total Thickness)

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  • Discrepancies in the determination of sperm

    Determination of sperm concentration by use of a haemocytometer or counting chamber is an important step in semen evaluation and is also used for calibration or validation of instruments.

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    2021-2-7 · 2.7 Sperm numbers 32 2.7.1 Types of counting chamber 34 2.7.2 The improved Neubauer haemocytometer 34 2.7.3 Using the haemocytometer grid 35 2.7.4 Care of the counting chamber 35 2.7.5 Fixative for diluting semen 36 2.7.6 Importance of counting suffi cient spermatozoa 36

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  • Estimating Sperm Concentration - Purdue University

    1999-8-17 · As with the other photometer devices, dirt, blood, sperm cell clumping and other debris can lead to an overestimate of sperm cell concentration. Hemocytometer The hemocytomter or counting chamber is a direct method of counting sperm cells. However it is an impractical and time-consuming method for use in routine semen laboratory operations.

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    2021-3-31 · Also, it offers more testing slides in one kit, each slide using two counting chambers with the possibility of testing in duplicate, suggesting an enhanced accuracy. Yet, to endorse the expected results with the ExSeed ® device, a clinical validation study comparing it to manual or automated sperm count …

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  • Normal sperm count: A guide to semen analysis

    2019-3-28 · A sperm count is part of a larger test called semen analysis. A doctor may test a person’s sperm count if they have fertility concerns. Learn about average, low, and high sperm counts here.

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  • Temporal trends in sperm count: a systematic review

    Sperm count is of considerable public health importance for several reasons. First, sperm count is closely linked to male fecundity and is a crucial component of semen analysis, the first step to identify male factor infertility (World Health Organization, 2010; Wang and Swerdloff, 2014).

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    sperm number was determined with a haemocytometer. Approximately, 10 lL of diluted sperm suspension was transferred to each counting chamber and was allowed to stand for 5 min and counting under a light microscope at 400· magnification. About 20 lL of sperm suspension was mixed with an equal volume of 0.05% eosin-Y and nigrosin. After 2-min

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    2020-8-13 · The Sperm Chromatin Structure Assay (SCSA®), in addition to identifying the DNA Fragmentation Index (DFI) also identifies High DNA satiability (HDS), supposed to reflect the nuclear compaction of spermatozoa. However, data on what exactly this parameter reveals, its relevance and usefulness are contradictory. In order to shed light on this situation, spermatozoa of a cohort (N = …

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  • Discrepancies in the determination of sperm

    Determination of sperm concentration by use of a haemocytometer or counting chamber is an important step in semen evaluation and is also used for calibration or validation of instruments.

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    2019-6-1 · Mercury is a widespread aquatic pollutant that adversely affects the reproductive system of male fish. Although the effects of mercuric chloride (HgCl 2) on the testicular structure, the sperm count and altered sperm morphology of fish have been investigated, the effects on sperm quality and fertilization are unknown.Therefore, the aim of this study was to investigate the effects of HgCl 2 ...

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    2019-12-19 · An aliquot of the capacitated sperm suspension was transferred to a prewarmed counting chamber (depth, 20 μm), and >200 sperm were examined …

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    2019-7-6 · The MFC design is divided into two parts, each having a unique function. The first part is the total sperm counting zone (Fig. 1a), where the sperms are injected and counted to determine their total number. The second part illustrated in Fig. 1b, c has the function of sorting motile and healthy sperms after media and sperm injection. In particular, Fig. 1a illustrates a common microfluidic ...

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    2016-3-24 · Also, no relevant changes in testicular weight (110±4 mg in wild-type and 104±6 mg in dKO), epididymal weight (37±1 mg in wid-type and 36±2 mg in dKO) and sperm count (1.3±0.2 × 10 6 /ml in ...

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    2018-11-1 · Regarding sperm quality of European eels, this is the first study that takes into account the effect of different origins of stimulated males (wild-caught compared with farmed) on sperm quality, and these findings may help to improve the production of high-quality gametes in this endangered species.

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    2012-3-13 · Sperm motility monitored by rotation and movement of glycerinated eggs. (A) Moving distances from the starting points (Left) to the ending points (Right) during a period of 1 s after the addition of sperm to the glycerinated eggs were measured and used as criteria of sperm binding and movement.(Scale bar, 200 μm.) (B) Moved distances of glycerinated eggs by sperm during a period of …

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    2010-12-7 · In the journey from the male to female reproductive tract, mammalian sperm experience a natural osmotic decrease (e.g., in mouse, from ∼415 mOsm …

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    2019-7-6 · The MFC design is divided into two parts, each having a unique function. The first part is the total sperm counting zone (Fig. 1a), where the sperms are injected and counted to determine their total number. The second part illustrated in Fig. 1b, c has the function of sorting motile and healthy sperms after media and sperm injection. In particular, Fig. 1a illustrates a common microfluidic ...

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    2013-8-23 · dia.). The Type 10 Micrometer features a scale of 5mm/100mm (20mm dia.). The

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    2018-1-17 · Reductions in sperm quality (e.g. sperm motility, viability) due to elevated body temperatures have been linked to infertility in poultry [12,48] and a range of mammalian species . Our findings suggest extreme environmental temperatures may result in a limited supply of functional sperm, and thus may also impact male fertility in passerine birds.

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    2016-3-21 · Objective . To analyze DNA methylation levels between two groups of spermatozoa taken from the same sample, following morphological selection by high magnification (HM) at 6100x microscopy. A prospective study was conducted and studied 876 spermatozoa from 10 randomly selected men. Sperm morphology was characterized at HM according to criteria previously established.

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    Get tips and helpful information for setting up of your cell counting or cell imaging experiment using our Countess™ automated cell counters and EVOS™ cell imaging systems. Also find support for getting started with high-content screening (HCS)/high-content analysis (HCA) using the CellInsight™ CX5, CellInsight™ CX7, and ArrayScan™ Systems.

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    Long-chain fatty acid β-oxidation is an important energy source during epididymal maturation of spermatozoa. Kuang et al. show that Slc22a14 is a riboflavin transporter located at the inner mitochondrial membrane. Slc22a14 deficiency in the spermatozoa mid-piece disrupts the riboflavin transport and subsequently alters flavoenzyme-mediated bioenergetic metabolism, resulting in male …

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    2011-11-17 · C. elegans male sperm activation is regulated by a protease. In wild-type males, sperm are stored in the inactive form within the seminal vesicle and become activated after transfer to a hermaphrodite (and unpublished data).Mutations in the secreted protease inhibitor SWM-1 result in premature sperm activation within males (Figure 1B, 1A, ).We predicted that loss of activation …

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  • Sperm Motility | Understand your Semen Analysis

    2019-10-1 · Overview of experiments investigating thermal adaptation and plasticity in sperm and egg biology and its adaptive significance. (a) Set up of long-term thermal selection lines (TSLs) maintained at 30°C or 38°C for 50+ non-overlapping generations with eight replicate populations per regime.(b) Experimental design to investigate gamete size divergence in 30°C and 38°C TSLs at their long-term ...

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    2020-6-2 · The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable for sperm-egg membrane fusion: the sperm proteins IZUMO1 and SPACA6, and the egg protein JUNO. Here we demonstrate that another sperm protein, TMEM95, is necessary for ...

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    Get tips and helpful information for setting up of your cell counting or cell imaging experiment using our Countess™ automated cell counters and EVOS™ cell imaging systems. Also find support for getting started with high-content screening (HCS)/high-content analysis (HCA) using the CellInsight™ CX5, CellInsight™ CX7, and ArrayScan™ Systems.

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  • Cell Counting Support—Getting Started | Thermo

    2018-1-17 · Reductions in sperm quality (e.g. sperm motility, viability) due to elevated body temperatures have been linked to infertility in poultry [12,48] and a range of mammalian species . Our findings suggest extreme environmental temperatures may result in a limited supply of functional sperm, and thus may also impact male fertility in passerine birds.

    Get Price
  • Experimental heatwaves negatively impact sperm

    2018-2-27 · Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the …

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    2018-6-1 · Potassium is a key component of cyprinid seminal plasma, 20,32,46 and incubation of sperm in a medium that contains potassium has been shown to increase and regenerate sperm motility. 21,22,32,47 Because of its beneficial characteristics, we tested and increased the amount of potassium in E400 (130 mM) compared to previous extender solutions ...

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    2007-12-1 · Using a microscope mounted with a heated stage (37°C), the sperm cell motility was determined by counting the proportion of a) fast progressive sperm; b) slowly progressive sperm; c) local motile sperm; and d) immotile sperm on 100 sperm within each of two fresh drops of semen, placed on a preheated (37°C) clean glass slide, and covered with ...

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    2011-5-1 · Sperm volume was measured by aspiration into a 10-mL pipette, providing 0.1-mL accuracy. Sperm concentration was determined using a micro cell as a counting chamber. Sperm motility was assessed at 20× magnification on the heating stage of a microscope (37°C), and spermatozoa were scored in categories A, B, C, and D.

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